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Potato is attacked by various fungal pathogens. Mycobiota associated with diseased bittersweet nightshade (Solanum dulcamara L.) and potato (Solanum tuberosum L.) leaves has been investigated by rDNA cloning. In total, 33 fungal species belonging to 26 genera have been identified. Fungal species from leaves of S. dulcamara included Alternaria alternata, Aureobasidium pullulans, Boeremia exigua, Botrytis cinerea, Cladosporium cladosporioides, C. herbarum, C. tenuissimum, Colletotrichum acutatum, C. gloeosporioides, Coniothyrium fuckelii, Fusarium oxysporum, Phoma herbarum, Phytophthora infestans, Thanatephorus cucumeris, and Thielavia basicola. Fungal species detected on S. tuberosum leaves included Alternaria alternata, A. solani, A. infectoria, Cladosporium cladosporioides, C. herbarum, C. tenuissimum, Colletotrichum coccodes, C. gloeosporioides, Epicoccum nigrum, Fusarium sp., Mycosphaerella sp., Phoma sp., Phytophthora infestans, Rhodotorula glutensis, Tolypocladium inflatum, Saccharomyces sp., Cryptococcus sp., C. tephrensis, Dioszegia crocea, and Hannaella oryzae. Mycobiota of healthy S. tuberosum leaves was studied as the control and included A. alternata, Aureobasidium pullulans, Cladosporium cladosporioides, C. herbarum, Cryptococcus wieringae, Hymenoscyphus scutula, Mycocentrospora acerina, Phaeohelotium epiphyllum,Phaeosphaeria culmorum, Plectosphaerella cucumerina, and Zalerion arboricola.