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Thermal acclimation and seasonal acclimatization: a comparative study of cardiac response to prolonged temperature change in shorthorn sculpinстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 сентября 2019 г.
- Авторы: Filatova Tatiana S., Abramochkin Denis V., Shiels Holly A.
- Журнал: Journal of Experimental Biology
- Том: 222
- Год издания: 2019
- Издательство: The Company of Biologists Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: jeb.202242
- DOI: 10.1242/jeb.202242
- Аннотация: Seasonal thermal remodelling (acclimatization) and laboratory thermal remodelling (acclimation) can induce different physiological changes in ectothermic animals. As global temperatures are changing at an increasing rate, there is urgency to understand the compensatory abilities of key organs like the heart to adjust under natural conditions. Thus, the aim of the present study was to directly compare the acclimatization and acclimatory response within a single eurythermal fish species, the European shorthorn sculpin (Myoxocephalus scorpio L.). We used current- and voltage-clamp to measure ionic current densities in both isolated atrial and ventricular myocytes at a common temperature of 7.5°C from three groups of fish: 1) summer-acclimated (SumAcl, 12°C); 2) cold-acclimated (ColdAcl, 3°C); and 3) winter-acclimatized fish (WinAcz, 3°C). At the common test temperature of 7.5°C, action potential (AP) was shortened by both WinAcz and ColdAcl, compared with SumAcl, however, WinAcz caused a greater shortening than did ColdAcl. Shortening of AP was achieved mostly by a significant increase in repolarizing current density (IKr and IK1) following WinAcz, with ColdAcl having only minor effects. Compared with SumAcl, the depolarizing L-type calcium current (ICa) was larger following WinAcz, but again there was no effect of ColdAcl on ICa Interestingly, the other depolarizing current, INa was downregulated at low temperatures. Our analysis further shows that ionic current remodelling is primarily due to changes ion channel density rather than currents kinetics. In summary, acclimatization profoundly modified the electrical activity of the sculpin heart while acclimation to the same temperature for >1.5 month produced very limited remodelling effects.
- Добавил в систему: Абрамочкин Денис Валерьевич