YB-1, an abundant core mRNA-binding protein, has the capacity to form an RNA nucleoprotein filament: a structural analysis

Kretov, D.A.; Clément, M.J.; Lambert, G.; Durand, D.; Lyabin, D.N.; Bollot, G.; Bauvais, C.; Samsonova, A.; Budkina, K.; Maroun, R.C.; Hamon, L.; Bouhss, A.; Lescop, E.; Toma, F.; Curmi, P.A.; Maucuer, A.; Ovchinnikov, L.P.; Pastré, D.

Статья опубликована в высокорейтинговом журнале

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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 апреля 2022 г.

Авторы: Kretov D.A., Clément M.J., Lambert G., Durand D., Lyabin D.N., Bollot G., Bauvais C., Samsonova A., Budkina K., Maroun R.C., Hamon L., Bouhss A., Lescop E., Toma F., Curmi P.A., Maucuer A., Ovchinnikov L.P., Pastré D.
Журнал: Nucleic Acids Research
Том: 47
Номер: 6
Год издания: 2019
Издательство: Oxford University Press
Местоположение издательства: United Kingdom
Первая страница: 3127
Последняя страница: 3141
Аннотация: The structural rearrangements accompanying mRNA during translation in mammalian cells remain poorly understood. Here, we discovered that YB-1 (YBX1), a major partner of mRNAs in the cytoplasm, forms a linear nucleoprotein filament with mRNA, when part of the YB-1 unstructured C-terminus has been truncated. YB-1 possesses a cold-shock domain (CSD), a remnant of bacterial cold shock proteins that have the ability to stimulate translation under the low temperatures through an RNA chaperone activity. The structure of the nucleoprotein filament indicates that the CSD of YB-1 preserved its chaperone activity also in eukaryotes and shows that mRNA is channeled between consecutive CSDs. The energy benefit needed for the formation of stable nucleoprotein filament relies on an electrostatic zipper mediated by positively charged amino acid residues in the YB-1 C-terminus. Thus, YB-1 displays a structural plasticity to unfold structured mRNAs into extended linear filaments. We anticipate that our findings will shed the light on the scanning of mRNAs by ribosomes during the initiation and elongation steps of mRNA translation.
Добавил в систему: Лябин Дмитрий Николаевич

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YB-1, an abundant core mRNA-binding protein, has the capacity to form an RNA nucleoprotein filament: a structural analysisстатья Исследовательская статья

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