Use of thermal lensing for the determination of pyrogensстатья
Статья опубликована в высокорейтинговом журнале
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The conditions of spectrophotometric determination of lipopolysaccharides (LPS) by methylene blue dye were proposed. The limit of detection of LPS is 100 ng/ml. The conditions of spectrophotometric determination of LPS with Stains-all were proposed, the limit of detection of LPS is 500 ng/ml. The optimization of spectrophotometric determination of LPS by the reaction of 2-keto-3-deoxyoctonic acid with thiobarbituric acid in the presence of periodate and metaarsenite was made, the limit of detection is 800 ng/ml. The limit of detection by thermal lensing is 70 ng/ml. The thermal-lens method was successfully used for LPS determination in the endotoxin standard from E. coli. (C) 2003 American Institute of Physics.