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First report of Colletotrichum theobromicola on raspberryстатья Исследовательская статья
- Авторы: Tsvetkova Y.V., Elansky S.N.
- Журнал: Journal of Plant Pathology
- Том: 107
- Год издания: 2025
- Издательство: Societa Italiana di Patologia Vegetale
- Местоположение издательства: Italy
- Первая страница: 769
- Последняя страница: 770
- DOI: 10.1007/s42161-024-01778-x
- Аннотация: In 2020, necrosis lesions on the stem with dark brown conidiomata were found on the seedlings of raspberry (Rubus idaeus) planting material of Italian origin (the Tuscany region, province of Pistoia). Isolated cultures were brownish-olive after 3 d, then darkened, cottony air mycelium appeared. The colony was smooth with orange conidial mass visible on the surface; setae was not observed. Conidia, on average 18.78 ± 2.01 × 5.58 ± 0.36 μm, elongated cylindrical, rounded at both ends, hyaline, unicellular. DNA was extracted from isolate Col_4 and ITS, TUB2, CHS-1, HIS3, GAPDH, LSU regions were amplified using primers: ITS5/ITS4, TUB2Fd/TUB4Rd, CHS-79 F/CHS-354R, CYLH3F/CYLH3R, GDF/GDR, LR0R/LR7, respectively. The resulting sequences were submitted to GenBank (ITS– MT905224.1, TUB2– ON921089.1, CHS-1– ON921087.1, HIS3– ON921088.1, GAPDH– ON921086.1, LSU– ON909006.1). By comparing the obtained sequences with the NCBI database, 100% similarity was shown in the gene regions of GAPDH (JX009953.1), CHS-1 (KP642641.1), TUB2 (MN304883.1). For ITS and HIS3 regions, the BLAST search showed 99.66% matches as JX010285.1 and 99.47% to MH713420.1, respectively. A multilocus analysis using Neighbor-Joining method based on combined ITS, CHS-1, GAPDH and TUB2 sequences with the software MEGA X showed that the isolate clustered in clade with the C. theobromicola strain CBS124945 with high bootstrap value (97). For the pathogenicity test 1 ml of a suspension of conidia (2 × 105 conidia/ml) was applied to the surface of the plant material. As a control, parts of the plants with the application of sterile water were used. After 7d, necrosis was observed on all parts of inoculated plants, aerial mycelium was present on the berries and sporulation of fungal fruiting body was observed after 10 d, whereas no symptoms occurred on controls. Symptoms were similar to naturally infected material. Reisolated fungi from lesions were confirmed to be Colletotrichum theobromicola Delacr. based on growth type of colonies, morphology characteristics of acervuli and conidia and the sequences of regions described above, thus fulfilling Koch’s postulates. Raspberry anthracnose is a widespread disease all over the world, often caused by C. fioriniae, C. acutatum, C. rubicola, C. neorubicola (Damm et al. 2012; Liu et al. 2020). The following species have also been noted on raspberry: C. coccodes, C. gloeosporioides, C. godetiae, C. fructicola, C. lineola (Weir et al. 2012). To our knowledge, this is the first report of anthracnose of raspberry caused by C. theobromicola.
- Добавил в систему: Еланский Сергей Николаевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Coll_teob.pdf | Coll_teob.pdf | 580,5 КБ | 16 апреля 2025 [elansky] | |
| 2. | Разрешение на публикацию | 20241018_120513.jpg | 3,2 МБ | 18 октября 2024 [elansky] |