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Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencingстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 2 мая 2018 г.
- Авторы: Carlson Bradley A., Lee Byeong Jae, Tsuji Petra A., Copeland Paul R., Schweizer Ulrich, Gladyshev Vadim N., Hatfield Dolph L.
- Сборник: Methods in Molecular Biology
- Том: 1661
- Год издания: 2018
- Место издания: Springer New York
- Первая страница: 43
- Последняя страница: 60
- DOI: 10.1007/978-1-4939-7258-6_4
- Аннотация: The selenocysteine (Sec) tRNA[Ser]Sec population consists of two isoforms that differ from each other by a single 2'-O-methylribosyl moiety at position 34 (Um34). These two isoforms, which are encoded in a single gene, Trsp, and modified posttranscriptionally, are involved individually in the synthesis of two subclasses of selenoproteins, designated housekeeping and stress-related selenoproteins. Techniques used in obtaining these isoforms for their characterization include extraction of RNA from mammalian cells and tissues, purifying the tRNA[Ser]Sec population by one or more procedures, and finally resolving the two isoforms from each other. Since some of the older techniques for isolating tRNA[Ser]Sec and resolving the isoforms are used in only a few laboratories, these procedures will be discussed briefly and references provided for more detailed information, while the more recently developed procedures are discussed in detail. In addition, a novel technique that was developed in sequencing tRNA[Ser]Sec for identifying their occurrence in other organisms is also presented.
- Добавил в систему: Гладышев Вадим Николаевич